This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of allantoin on ACQUITY UPLC BEH Amide Columns.
            
        | 
                     Column:  | 
                     ACQUITY UPLC BEH Amide, 2.1 x 150 mm, 1.7 μm  | 
            
| 
                     Part Number:  | 
                     186004802  | 
            
| 
                     Isocratic Mobile Phase B:  | 
                     90/10 MeCN/H2O  | 
            
| 
                     Flow Rate:  | 
                     0.2 mL/min  | 
            
| 
                     Injection Volume:  | 
                     5.0 μL (PLNO)  | 
            
| 
                     Sample Concentration:  | 
                     100 μg/mL  | 
            
| 
                     Sample Diluent:  | 
                     90/10 MeCN/H2O  | 
            
| 
                     Column Temperature:  | 
                     25 °C  | 
            
| 
                     Weak Needle Wash:  | 
                     95/5 MeCN/H2O  | 
            
| 
                     Detection:  | 
                     UV @ 210 nm  | 
            
| 
                     Sampling Rate:  | 
                     20 points/sec  | 
            
| 
                     Filter Time Constant:  | 
                     0.2  | 
            
| 
                     Instrument:  | 
                     Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector  | 
            
The compound used in this study is:
            
        WA60107, June 2009