This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of herbicides used in weed control.
Compounds used in this study:
1. Atrazine-desethyl-desisopropyl
2. Picloram
3. Chlorsulfuron (unknown impurity)
4. Atrazine
5. 2,4-D
            
        | 
                     Column:  | 
                     SunFire C18 4.6 x 150 mm, 5 μm (p/n: 186002559)  | 
            
| 
                     Mobile phase A:  | 
                     Water  | 
            
| 
                     Mobile phase B:  | 
                     Acetonitrile  | 
            
| 
                     Mobile phase C:  | 
                     100 mM CH3COO-NH4 + pH 4.5  | 
            
| 
                     Flow rate:  | 
                     1 mL/min  | 
            
| 
                     Injection volume:  | 
                     10 μL  | 
            
| 
                     Sample concentration:  | 
                     4 μg/mL in water Chlorsulfuron 8 μg/mL (2,4-Dichlorophenoxy)acetic Acid 40 μg/Ml  | 
            
| 
                     Temperature:  | 
                     30 °C  | 
            
| 
                     Detection:  | 
                     UV @ 220 nm  | 
            
| 
                     Instrument:  | 
                     Alliance 2695 with 2996 PDA  | 
            
            
        
            
        WA41891, May 2005