• Application Note

Sulfa Drugs in Serum

Sulfa Drugs in Serum
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of sulfa drugs in serum using Symmetry columns.

Introduction

Compounds used in this study are:

1: Sulfadiazine

2: Sulfathiazole (I.S.)

3. Sulfamerazine

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm (p/n: WAT046980)

Mobile phase:

1% glacial acetic acid and 4% methanol in water

Flow rate:

1.0 mL/min

Injection volume:

10 μL of reconstituted porcine serum extract

Temperature:

25° C

Detection:

UV @ 254 nm

Results and Discussion

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WA31763.155, June 2003

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