• Application Note

Separation of a Tryptic Digest of Human Serum Albumin

Separation of a Tryptic Digest of Human Serum Albumin
  • Waters Corporation
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Human serum albumin molecule structure

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates separation of a tryptic digest of human serum albumin.

Introduction

The compound used in this study is tryptic digest of human serum albumin.

Experimental

Column:

BioSuite C18 3 μm PA-A (2.1 x 250 mm)

Eluent A:

0.1% Formic acid in water

Eluent B:

0.1% Formic in acetonitrile

Gradient:

0–40% B in 300 min

Flow rate:

0.16 mL/min

Injection volume:

0.2 mL/min

Temperature:

40 °C

Detection:

UV 220 nm

Results and Discussion

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WA31793, June 2003

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