Flavonoids in Ginkgo

This application brief demonstrates analysis of flavonoids in ginkgo.

The compounds used in this study are –

1. Quercetin
2. Kaempferol
3. Isorhamnetin

Sample Pre-Preparation

1 g sample is refluxed in 50 mL of ethanol: 3M HCl (70:30) for 2.5 hr

The cooled sample is adjusted to exactly 100 mL 

0.3 mL of the ethanolic extract is diluted 1:10 with water

• Recovery, measured with certified standards at 100 ppm in reagent water, was 82% for quercitin and >90% for the other compounds
• All analyses gave results within ±40% of the expected values with the exception of the capsule (+60%)
• The selective SPE extraction and cleanup procedure provided a convenient analysis of ginkgo flavonoids in a complex matrix (Herbal One with 16 herbal ingredients)