Multi-Residue Analysis of Pesticides in Grapes Using AOAC QuEChERS Method by GC-MS

This application brief demonstrates multi-residue analysis of pesticides in grapes using AOAC QuEChERS method by GC-MS.

Extraction Procedure

1. Add 15 mL 1% acetic acid in acetonitrile into the 50 mL DisQuE extraction tube 1.
2. Add 15 g of homogenized sample into the 50 mL tube.
3. Add any internal standards and standard mixture.
4. Shake vigorously for 1 minute and centrifuge > 1500 rcf for 5 minute.
5. Transfer 1 mL of the acetonitrile extract into the clean-up tube 2.
6. Shake for 30 seconds and centrifuge >1500 rcf for 1 minute.
7. Transfer 0.5 mL extract into a tube.
8. Add any post-extraction internal standards.
9. Add 0.25 mL toluene.
10. Evaporate at 50 °C with N₂ to < 0.1 mL.
11. Bring volume up to 0.2 mL with toluene.
12. Transfer to vial with insert for analysis.