• 应用纪要

ACQUITY UPLC Analysis of 5-Fluorouracil

ACQUITY UPLC Analysis of 5-Fluorouracil

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates AQUITY UPLC analysis of 5-Fluorouracil.

Introduction

Structure

Experimental

Test Conditions

Columns:

ACQUITY UPLC BEH Amide, 2.1 x 50 mm, 1.7 μm

Part Number:

186004800

Isocratic Mobile Phase:

95/2.5/2.5 MeCN/IPA/H2O with 5 mM CH3COONH4 and 0.02% NH4OH, pH 9.0

Flow Rate:

0.2 mL/min

Injection Volume:

5.0 μL (PLNO)

Sample Concentration:

50 μg/mL

Sample Diluent:

75/25 MeCN/MeOH with 0.2% HCOOH

Column Temperature:

25 °C

Weak Needle Wash:

95/5 MeCN/H2O

Detection:

UV @ 265 nm

Sampling Rate:

20 points/sec

Filter Time Constant:

0.2

Instrument:

Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector

Results and Discussion

WA60092, June 2009

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