• 应用纪要

EPA Method 8310.0 Determination of Polycyclic Aromatic Hydrocarbons in Ground Water and Wastes

EPA Method 8310.0 Determination of Polycyclic Aromatic Hydrocarbons in Ground Water and Wastes
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the determination of Polycyclic aromatic hydrocarbons in ground water and wastes.

Introduction

Polycyclic aromatic hydrocarbons (PAHs) are one of the most widespread organic pollutants. PAHs are made up of fused aromatic rings and are formed during the combustion of carbon-based fuels (wood, coal, diesel), as well as being present in crude oil. The United States Environmental Protection Agency (US EPA) has classified seven PAH compounds as being potentially carcinogenic including benz[a]anthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene, chrysene, dibenz[a,h]anthracene, and indeno[1,2,3-cd]pyrene.

Experimental

HPLC Conditions

Instrument:

Waters Alliance HPLC System with PDA and Fluorescence Detectors

Eluent:

Water/Acetonitrile

Column:

Waters PAH 4.6 x 250 mm @ 30 °C

Injection:

20 μL of Supelco standard EPA 610 (#48743) diluted 1:50 in 40:60 water/acetonitrile

Flow rate:

1.2 mL/min

Detection:

UV @ 254 nm and fluorescence using timed programmed wavelengths

Data:

Waters Empower Software

Sample Preparation

MeCl2 extraction.

Eluent Preparation

Filter and degas through a 0.45 μm filter.

A: Water

B: Acetonitrile

Eluent gradient.

1 Fluorescence mode used for detection limit determination, no pre concentration. Seven replicates per 40 CFR pt. 136 App. B.

2 Regulated compound; action level 0.17 ppb.

PAH target analytes.
Standard chromatogram, UV @ 254 nm, 1–20 ppm PAH analytes.
Standard chromatogram, fluorescence/programmed wavelengths, 1-20 ppm PAH analytes.

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