This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of caffeine and metabolites using XBridge C18 Columns.
|
Column: |
XBridge C18, 5 μm, 4.6 x 150 mm p/n: 186003116 |
|
Mobile Phase A: |
5 Mm ammonium acetate pH 7.5 |
|
Mobile Phase B: |
acetonitrile |
|
Flow Rate: |
1.0 mL/min |
|
Injection Volume: |
10 μL |
|
Sample Concentration: |
20 μg/mL in water |
|
Temperature: |
25 ˚C |
|
Detection: |
UV @ 273 nm |
|
Instrument: |
Alliance 2695 with 2996 PDA |
|
Time (min) |
%A |
%B |
|---|---|---|
|
0.0 |
98 |
2 |
|
15.0 |
80 |
20 |
|
18.0 |
80 |
20 |
|
19.0 |
98 |
2 |
|
25.0 |
98 |
2 |
WA60169, April 2007