This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of phenones.
The compounds used in this study are –
            
        | 
                     Column:  | 
                     Atlantis dC18, 2.1 x 20 mm IS, 3 μm, (P/N: 186002058) Symmetry Shield RP18, 2.1 x 20 mm IS,3.5 μm, (P/N:186002068) Symmetry C18, 2.1 x 20 mm IS, 3.5 μm, (P/N: 186002066) Xterra MS C18, 2.1 x 20 mm IS, 3.5 μm,(P/N: 186001923)  | 
            
| 
                     Mobile phase A:  | 
                     0.1% HCOOH in Water  | 
            
| 
                     Mobile phase B:  | 
                     0.1% HCOOH in Acetonitrile  | 
            
| 
                     Flow Rate:  | 
                     0.6 mL/min  | 
            
| 
                     Injection Volume:  | 
                     5 μL  | 
            
| 
                     Sample concentration:  | 
                     20 μg/mL  | 
            
| 
                     Temperature:  | 
                     30 °C  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
| 
                     Instrument:  | 
                     Alliance 2795 with 996 PDA  | 
            
            
        
            
        WA31787.22, June 2003