 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of hydrocortisone and related compound.
The compounds used in this study are –
| Compounds | USP Tailing Factors | 
|---|---|
| Hydrocortisone | 1.1 | 
| Hydrocortisone-21-Acetate | 1.1 | 
 
            
        | Column: | Symmetry C18, 3.9 x 150 mm, 5 μm | 
| Part number: | WAT046980 | 
| Mobile phase: | Acetonitrile/water 35:65 | 
| Flow rate: | 1.0 mL/min | 
| Injection volume: | 10 μL of 10 μg/mL hydrocortisone and 30 μg/mL hydrocortisone-21-acetate | 
| Detection: | UV @ 254 nm | 
 
            
        WA31763.89, June 2003