• 应用纪要

Chlorzoxazone in Rat Plasma

Chlorzoxazone in Rat Plasma
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of chlorzoxazone in rat plasma using Oasis MAX extraction method.

Introduction

Chlorzoxazone is analyzed in this application brief.

Experimental

HPLC Method

Column:

XTerra MS C18 4.6 x 30 mm, 3.5 μm (p/n: 186000398)

Mobile phase A:

0.2% HCOOH

Mobile phase B:

ACN

Isocratic mobile phase composition:

45% A; 55% B

Flow rate:

0.2 mL/min

Injection volume:

20 μL

Temperature:

60 °C

Detection:

MS ESI-

Instrument:

Alliance 2790, Micromass Quattro Ultima

MS Conditions

Ion source:

ESI-

Source temp.:

150 °C

Gas cell:

1.5e-3 mbar, 20 eV

Desolvation temp.:

350 °C

Cone gas flow:

150 L/hr

Drying gas flow:

600 L/hr

Cone voltage:

30 V

Results and Discussion

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WA31764.53, June 2003

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