This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of betamethasone.
The compounds used in this study are –
| 
                     Compounds  | 
                     USP Tailing Factors  | 
            
|---|---|
| 
                     Betamethasone  | 
                     1.12  | 
            
| 
                     Propylparaben (I.S.)  | 
                     1.09  | 
            
            
        | 
                     Column:  | 
                     Symmetry C8, 3.9 x 150 mm, 5 μm  | 
            
| 
                     Guard column:  | 
                     Sentry Guard Column 3.9 x 20 mm, 5 μm  | 
            
| 
                     Part numbers:  | 
                     Column - WAT046970, Guard - WAT054250  | 
            
| 
                     Mobile phase:  | 
                     Water/acetonitrile/phosphoric acid 63:37:0.1  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     10 μL of 50 μg/mL sample  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
            
        WA31763.37, June 2003