• Note d'application

Multi-Residue Analysis of Pesticides in Flour Using AOAC QuEChERS Method by UPLC-MS/MS

Multi-Residue Analysis of Pesticides in Flour Using AOAC QuEChERS Method by UPLC-MS/MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates multi-residue analysis of pesticides in flour using AOAC QuEChERS method by UPLC-MS/MS.

Experimental

Extraction Procedure

  1. Add 15 mL 1% acetic acid in acetonitrile into the 50 mL DisQuE extraction tube 1.
  2. Diluted 5 g flour with 10 mL water and soak for 10 min.
  3. Add sample into the 50 mL tube.
  4. Add any internal standards and standard mixture.
  5. Shake vigorously for 1 minute and centrifuge > 1500 rcf for 5 minute.
  6. Transfer 1 mL of the acetonitrile extract into the clean-up tube 2.
  7. Shake for 30 seconds and centrifuge >1500 rcf for 1 minute.
  8. Transfer 100 μL of final extract into an autosampler vial.
  9. Add any post-extraction internal standards.
  10. Dilute as needed with an appropriate buffer or solvent.

Test Conditions

LC Conditions

LC System:

Waters ACQUITY UPLC System

Column:

ACQUITY UPLC BEH C18, 2.1 x 100 mm, 1.7 μm

Column Temp:

40 °C

Sample Temp:

4 °C

Flow Rate:

0.3 mL/min.

Mobile Phase A:

Water + 0.1% formic acid

Mobile Phase B:

Methanol + 0.1% formic acid

Injection Volume:

15 μL, Partial loop injection

Gradient:

Time

Flow Rate

A%

B%

0.00

0.3

75

25

0.25

0.3

75

25

7.75

0.3

5

100

8.50

0.3

0

100

8.51

0.5

75

25

10.50

0.5

75

25

11.0

0.3

75

25

MS Conditions

Instrument:

Waters ACQUITY TQ Detector

Ionization:

Positive electrospray (ESI+)

Acquisition:

Multiple reaction monitoring (MRM)

Results and Discussion

Pesticide Recovery in Flour by UPLC-MS/MS
720003371en-f1

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720003371, March 2010

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