ACQUITY UPLC HILIC Gradient Separation of Organophosphonic Acids

Columns:

ACQUITY UPLC BEH Amide, 2.1 x 100 mm, 1.7 μm

Part Number:

186004801

Mobile Phase A:

50/50 MeCN/H₂O with 10 mM CH₃COONH₄and 0.04% NH₄OH, pH 9.0

Mobile phase B:

95/5 MeCN/H₂O with 10 mM CH₃COONH₄ and 0.04% NH₄OH, pH 9.0

Flow Rate:

0.5 mL/min

Injection Volume:

5.0 μL (PLNO)

Sample Concentration:

2 μg/mL each

Sample Diluent:

75/25 MeCN/MeOH

Column Temperature:

65 °C

Weak Needle Wash:

95/5 MeCN/H₂O

Instrument:

Waters ACQUITY UPLC with ACQUITY SQD

Ionization Mode:

ES-

Capillary:

2.5 KV

Cone:

30 V (EMPA, IMPA, PMPA);

40 V (CMPA); 35 V (MMPA)

Source Temperature:

120 °C

Desolvation Temperature:

400 °C

Desolvation Gas Flow:

800 L/Hr

Cone:

5 L/Hr

SIR m/z:

122.9 (EMPA); 136.95 (IMPA);

179.0 (PMPA); 177.0 (CMPA);

150.95 (MMPA)

Dwell Time:

0.1 s