• Note d'application

Analysis of DNPH Derivatives Using XBridge Phenyl 

Analysis of DNPH Derivatives Using XBridge Phenyl 
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of DNPH derivatives using XBridge Phenyl columns.

Introduction

Regulatory agencies around the world are interested in measuring formaldehyde and other aldehydes in the air. Many public health groups are interested in implications of these aldehydes causing respiratory irritation and potentially carcinogenic effects from prolonged exposure. Producers of products that can contribute aldehydes emissions to air and indoor pollutants are manufacturers of materials building and wood products, fabric and textiles, and automotive companies.

Experimental

Test Conditions

Columns:

XBridge Phenyl, 4.6 x 100 mm, 3.5 μm p/n: 186003334

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

0.2% HCOOH in H2O

Flow rate:

1.2 mL/min

Injection volume:

10 μL

Sample:

Acetaldehyde-DNPH (10 μg/mL),

Acetone-DNPH (10 μg/mL),

Cyclohexanone-DNPH (10 μg/mL),

Formaldehyde-DNPH (10 μg/mL),

Crotonaldehyde-DNPH (10 μg/mL) in H2O/ACN (60/40)

Column temp.:

30 °C

Sample temp.:

15 °C

Detection:

UV @ 254 nm

Sampling Rate:

5 points/sec

Filter Response:

0.2

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time (min)

%A

%B

%C

0.00

40

50

10

2.67

40

50

10

6.67

0

90

10

7.33

40

50

10

11.00

40

50

10

Results and Discussion

Compounds: 1. Formaldehyde -DNPH 2. Acetaldehyde -DNPH 3. Acetone -DNPH 4. Crotonaldehyde -DNPH 5. Cyclohexanone -DNPH.

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WA60186, June 2007

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