• Note d'application

Testosterone Esters in Serum

Testosterone Esters in Serum

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of testosterone esters in serum.

Introduction

The compounds used in this study are – 

  1. Testosterone acetate
  2. Testosterone propionate
  3. Testosterone benzoate
  4. Testosterone enanthate (I.S.)

Experimental

HPLC Method

Column:

Symmetry C8, 4.6 x 75 mm, 3.5 μm

Guard column:

Sentry Guard Column, 3.9 x 20 mm, 5 μm

Part numbers:

Column - WAT066224, Guard - WAT054225

Mobile phase:

Acetonitrile/water 60:40

Flow rate:

1.5 mL/min

Injection volume:

20 μL of reconstituted porcine serum extract

Detection:

UV @ 254 nm

Results and Discussion

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample

Featured Products

WA31763.166, June 2003

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