• Note d'application

5’ – HEX Labeled 25mer Oligonucleotide Purification (0.1 μmole)

5’ – HEX Labeled 25mer Oligonucleotide Purification (0.1 μmole)
  • Waters Corporation
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Oligonucleotide illustration

Abstract

This application brief highlights the oligonucleotide purification using XTerra columns.

Introduction

5’ – HEX Labeled 25mer Oligonucleotide are analzed in this application brief.

Experimental

HPLC Method

Column:

XTerra MS C18 4.6 x 50 mm, 2.5 μm (p/n: 186000602)

Mobile phase A:

5% ACN, 95% 100 mM Triethylammonium acetate, pH 7.0

Mobile phase B:

30% ACN, 70% 100 mM Triethylammonium acetate, pH 7.0

Flow rate:

1.0 mL/min

Injection volume:

50 μL

Temperature:

60°C

Detection:

UV @ 260 nm

Instrument:

Alliance 2695, 2996 PDA

Gradient

Time (min)

%A

%B

0

100

0

15

0

100

Results and Discussion

Featured Products

WA20738.001, June 2002

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