• Applikationsbericht

Nalidixic Acid Antibiotics by LC-MS

Nalidixic Acid Antibiotics by LC-MS
  • Waters Corporation
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Red and white antibiotic capsules

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of nalidixic acid antibiotics by LC-MS.

Introduction

The compounds used in this study are – 

Experimental

LC Conditions

Column:

Atlantis dC18, 2.1 x 20 mm IS, 3.0 μm, (p/n: 186002058)

Mobile phase A:

Water

Mobile phase B:

Methanol

Mobile phase C:

1% HCOOH in Water

Flow rate:

0.4 mL/min

Injection volume:

2 μL

Sample concentration:

10 μg/mL

Temperature:

30 °C

Instrument:

Alliance 2795 and Waters ZQ

Gradient

Gradient.

MS Conditions

Waters ZQ

ES+ capillary (kV):

3.5

Cone (V):

5

Extractor:

3

RF lens:

0.1

Source temp. (°C):

150

Desolvation temp. (°C):

400

Cone gas flow (L/Hr):

50

Desolvation gas fLow(L/Hr):

500

LM resolution:

15

HM resolution:

15

Ion energy:

0.5

Multiplier (V):

650

Results and Discussion

The top figure is the total ion current, followed by the extracted ion signals for each of the three analytes.

*The “extra” peak in the cinoxacin panel is the isotope from oxolinic acid.

Featured Products

WA31787.18, June 2003

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