• Applikationsbericht

Morphine and Its Glucuronide Metabolites in Serum

Morphine and Its Glucuronide Metabolites in Serum

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of morphine and its glucuronide metabolites in serum.

Introduction

The compounds used in this sudy are - 

  1. Morphine-D3-glucuronide
  2. Morphine-D6-glucuronide
  3. Morphine

Experimental

HPLC Method

Column:

Symmetry Shield RP8, 3.9 x 150 mm, 5 μm

Part number:

WAT200655

Mobile phase:

20 mM potassium phosphate, pH 6.4

Flow rate:

1.0 mL/min

Injection volume:

100 μL of porcine serum extract

Detection:

Fluorescence; ex 280 nm, em 355 nm

Results and Discussion

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample

Featured Products

WA31763.104, June 2003

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