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Alkaloids in Goldenseal - Commercial Products or Whole Leaf

Alkaloids in Goldenseal - Commercial Products or Whole Leaf
  • Waters Corporation
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Goldenseal or Hydrastis canadensis growing on farm

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of alkaloids in goldenseal. 

Introduction

The compound analyzed in this study is berberine. 

Berberine

Experimental

HPLC Conditions

Column:

Symmetry C18, 4.6 x 250 mm, 5 µm

Part number:

WAT054275

Mobile phase A:

0.1% phosphoric acid

Mobile phase B:

Acetonitrile

Flow rate:

1.5 mL/min

Injection volume:

10 µL

Temperature:

35 °C

Detection:

UV @ 330 nm

Gradient

Gradient
Oasis MCX Extraction Method
Sample

Sample Pre-preparation

100 mg sample is extracted with 25 mL of ethanol:water (70:30) 1 mL of the ethanolic extract is diluted 1:3 with water (~ pH 7)

  • Recovery, measured with certified standards at 100 ppm in reagent water, was greater than 85 % for all compounds
  • All analyses gave results within ± 35 % of the expected values with the exception of the liquid supplement (see goldenseal analysis)
  • The selective SPE extraction and cleanup procedure provided a convenient analysis of echinacea phenolics in complex matrix such as in herbal tea 

Results and Discussion

Results

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WA31764.39, June 2003

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