• Note d'application

Tetracyclines in Honey

Tetracyclines in Honey
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief describes the methods to determine the presence of tetracyclines in honey.

Introduction

Tetracyclines (TCs), an antibiotic, is not permitted in apiculture. This method can monitors the presence of TC’s in honey.

Experimental

Pre-treatment

1. Add 30 mL EDTA-McIlvaines buffer to 6 g sample, mix thoroughly for 1 minute.

2. Centrifuge at 3000 rpm for 5 minutes and collect supernatant for SPE.

SPE Procedure

Solutions

McIlvaines Buffer

  1. Thoroughly mix 1000 mL 0.1 M citric acid with 625 mL 0.1 ML disodium hydrogen phosphate dihydrate.
  2. Adjust with sodium hydroxide or hydrochloric acid to pH 4 ± 0.05, if necessary.

EDTA-McIlvaines Buffer

  1. Add 60.5 g disodium EDTA to 1625 mL McIlvaines Buffer and mix thoroughly.

LC Conditions

Column:

SunFire C8, 3.5 μm, 2.1 x 150 mm

Mobile phase:

Acetonitrile:methanol: 0.4% fomic acid (18:4:78)

Flow rate:

0.2 mL/min

Injection volume:

20 μL

Column temp.:

25 ˚C

MS Conditions

MS System:

Waters Quadrupole MS

Ionization mode:

Positive electrospray (ESI+)

Multiple reaction monitoring
MRM method parameters.

Results and Discussion

Chromatogram of A. oxytetracycline, B. tetracycline, C. chlortetracycline and D. doxycycline standards.

References

  1. China GB/T 18932.23 - 2003.

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