• Note d'application

Analysis of Drugs of Abuse Using XBridge Shield RP18 

Analysis of Drugs of Abuse Using XBridge Shield RP18 
  • Waters Corporation
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For forensic toxicology use only.

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of drugs of abuse using Bridge Shield RP18 Columns.

Introduction

Benzodiazepines, such as clonazepam, bromazepam, lorazepam and flunitrazepam area class of drugs with sedative, hypnotic, anxiolytic,  anti-convulsant, amnestic, and muscle relaxant properties. They are  considered minor tranquilizers and have a high potential for abuse. MDA is the parent drug of MDMA, also known as Ecstasy. They are commonly  abused, amphetamine – derived recreational drugs.

Experimental

Test Conditions

Columns:

XBridge Shield RP18 4.6 X 100 mm, 3.5 μm p/n: 186003044

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM NH4HCO3, pH 9.6

Flow rate:

0.6 mL/min

Injection volume:

10 μL

Sample Concentration and Diluent:

10 μg/mL in H2O

Temp.:

40 °C

Sampling Rate:

5 points /second

Detection:

UV @ 210 nm

Time Constant:

1.0

Needle Wash:

5/95 MeOH/H2O

Instrument:

Alliance 2695 with 2996 PDA

System Suitability Parameters

Gradient

Time(min)

%A

%B

%C

0

63

32

5

3

63

32

5

7

45

50

5

9

45

50

5

10

63

32

5

12

63

32

5

Results and Discussion

Featured Products

WA60202, June 2007

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