• Note d'application

Antiepileptics and Metabolites

Antiepileptics and Metabolites
  • Waters Corporation
Télécharger le PDF Télécharger le PDF

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on the analysis of antiepileptics and its metabolites using Symmetry Columns. 

Introduction

The compounds analyzed in this study are:

  1. 2-Phenyl-2ethylmalonamide 
  2. Phenobarbital  
  3. p-Hydroxyphenobarbital 
  4. Carbamezapine-10,11-epoxide 
  5. Primidone 
  6. Carbamezapine 
  7. 10,11-Dihydrocarbamezapine 
  8. Phenytoin 

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 µm

Guard column:

Symmetry Guard Column 3.9 x 20 mm, 5 µm

Part numbers:

Column - WAT046970, Guard - WAT054250

Mobile phase:

100 mM potassium phosphate, pH 6.9/acetonitrile/methanol/water 17:25:5:53

Flow rate:

1.0 mL/min

Injection volume:

5 µL of 25µg/mL sample

Detection:

UV @ 214 nm

USP Tailing Factors

  1. NA
  2. NA
  3. 1.13
  4. 1.17
  5. 1.10
  6. 1.17
  7. NA
  8. NA

Results and Discussion

Featured Products

WA31763.30, June 2003

Haut de la page Haut de la page