This application brief demonstrates analysis of anesthetics using Atlantis T3 Columns.
The compunds used in this study are -
Column: |
Atlantis T3, 4.6 x 150 mm, 5 μm |
Part number: |
186003747 |
Mobile phase A: |
H2O |
Mobile phase B: |
MeOH |
Mobile phase C: |
100 mM HCOONH4, pH 3.75 |
Flow rate: |
1.4 mL/min |
Injection volume: |
5 μL |
Sample: |
Procaine (30 μg/mL), Lidocaine (100 μg/mL), Butacine (60 μg/mL) Benzocaine(30 μg/mL), Tetracaine (60 μg/mL) in H2O |
Column temperature: |
30 ˚C |
Sample temperature: |
15 ˚C |
Detection: |
UV @ 254 nm |
Sampling rate: |
5 point/sec |
Filter response: |
0.2 |
Instrument: |
Waters Alliance 2695 with 2996 PDA |
Time(min) |
Profile |
||
%A |
%B |
%C |
|
0.00 |
65 |
25 |
10 |
5.00 |
40 |
50 |
10 |
10.00 |
40 |
50 |
10 |
11.00 |
65 |
25 |
10 |
15.00 |
65 |
25 |
10 |
WA60218, January 2009