• Application Note

Analysis of Cephalosporin Antibiotics Using XBridge C18 

Analysis of Cephalosporin Antibiotics Using XBridge C18 
  • Waters Corporation
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Cefalexin antibiotic drug molecule

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of cephalosporin antibiotics using XBridge C18 Columns.

Introduction

Compounds used in this study. 

Experimental

Test Conditions

Column:

XBridge C18, 5 μm, 4.6 x 150 mm p/n: 186003116

Isocratic Mobile Phase:

0.5% acetic acid/acetonitrile (85:15)

Flow Rate:

1.0 mL/min

Injection Volume:

10 μL

Sample Concentration:

20 μg/mL in water

Temperature:

25 ˚C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Results and Discussion

Chromatogram
1. Cefadroxil 2. Cephradine 3. Cephalexin 4. Cefaclor.

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