• Application Note

Polar and Non-Polar Compounds Test Mix – 4.6 x 20 mm IS Separation 

Polar and Non-Polar Compounds Test Mix – 4.6 x 20 mm IS Separation 
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of polar and non-polar compounds test mix using Intelligent Speed Separation columns.

Introduction

Compounds used in this study are:

1. 8-Bromoguanosine
2. Acetanilide
3. Triamcinolone
4. Hydrocortisone
5. 2-Amino-7-chloro-5-oxo-5H-[1]benzopyrano[2,3-b]pyridine-3-carbonitrile
6. 6a-Methyl-17a-hydroxyprogesterone
7. 3-Aminofluoranthene
8. 2-Bromofluorene
9. Perylene
10. Naphtho[2,3-a]pyrene

Experimental

Conditions

Columns:

Atlantis dC18, 4.6 x 20 mm IS, 3 μm, (P/N: 186002062)

SymmetryShield RP18, 4.6 x 20 mm IS, 3.5 μm, (P/N: 186002092)

Symmetry C18, 4.6 x 20 mm IS, 3.5 μm, (P/N: 186002090)

XTerra MS C18, 4.6 x 20 mm IS, 3.5 μm, (P/N: 186001891)

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in Acetonitrile

Mobile phase C:

1% HCOOH in Water

Flow rate:

3.0 mL/min

Injection volume:

10 µL

Sample concentration:

20 µg/mL

Temperature:

30˚C

Detection:

UV @ 254 nm

Instrument:

Alliance 2795 with 996 PDA

Gradient

Results and Discussion

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WA31787.23, June 2003

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