• Application Note

Morphine and its Glucuronide Metabolite in Rat Plasma by Reverse-Phase SPE

Morphine and its Glucuronide Metabolite in Rat Plasma by Reverse-Phase SPE
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of morphine and its glucuronide metabolite using Osis HLB generic extraction method.

Introduction

Morphine and its glucuronide metabolite has been studied in rat plasme using Oasis HLB generic extraction method.

Experimental

LC Conditions

Column:

XTerra MS C18, 2.1 x 30 mm, 3.5 μm

Mobile phase A:

Acetonitrile + 0.1 M NH4HCO3 pH 9.7

Mobile phase B:

Water + 0.1 M NH4HCO3 pH 9.7

Flow rate:

0.2 mL/min

Temperature:

Ambient

LC:

Alliance 2795

Gradient

MS Conditions

MS:

Micromass Quattro Altima

Ion source:

Electrospray positive

Source temp.:

150 °C

Gas cell:

2.0 e-3 bar Argon

Desolvation temp.:

350 °C

Capillary voltage:

3.5 KV

Drying gas flow:

500 L/hr

Cone gas flow:

50 L/hr

Cone voltage:

35 V

MRM Transitions

Morphine:

m/z 285.8, 152.8 (40 collision)

Morphine-GLU:

m/z 462.0, 286.0 (25 collision)

Morphine-3D (I.S.):

m/z 288.9, 152.8 (40 collision)

Results and Discussion

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WA31764.98, June 2003

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