• Application Note

Tricyclic Antidepressants- Gradient, pH 9.0, XTerra RP18

Tricyclic Antidepressants- Gradient, pH 9.0, XTerra RP18
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the analysis of tricyclic antidepressants using XTerra RP18 Columns. 

Introduction

The compounds analyzed in this study are:  

  1. Nordoxepin   
  2. Desipramine   
  3. Nortriptyline   
  4. Doxepin   
  5. Imipramine   
  6. Amitriptyline   
  7. Trimipramine 
Compounds

Experimental

Conditions

Column:

XTerra RP18 4.6 x 50 mm, 3.5 µm

Part number:

186000434

Mobile phase A:

H2O

Mobile phase B:

MeOH

Mobile phase C:

100 mM NH4HCO3, pH 9.0

Flow rate:

2.0 mL/min

Injection volume:

20 µL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA

Gradient

Gradient

Results and Discussion

Results

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WA20738.106, June 2002

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