• Application Note

Tricyclic Antidepressants - Gradient, pH 3.8, XTerra Phenyl  

Tricyclic Antidepressants - Gradient, pH 3.8, XTerra Phenyl  
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on the analysis of tricyclic antidepressants using XTerra Columns.

Introduction

The compounds analyzed in this study are:

  1. Nordoxepin
  2. Doxepin 
  3. Impurity of Doxepin
  4. Desipramine
  5. Imipramine
  6. Amitriptyline
Compounds

Experimental

Connditions

Column:

XTerra Phenyl, 4.6 x 150 mm, 5 µm

Part number:

186001146

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM NH4COOH in H2O, pH 3.8

Flow rate:

0.7 mL/min

Isocratic mobile phase composition:

55% A; 35% B; 10% C

Injection volume:

10 µL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA

Results and Discussion

Results

Featured Products

WA20738.107, June 2002

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