ACQUITY UPLC Analysis of Morphine

Column:

ACQUITY UPLC BEH Amide, 2.1 x 50 mm, 1.7 μm

Part Number:

186004800

Mobile Phase A:

50/50 MeCN/H₂O with 10 mM NH₄COOH and 0.125% HCOOH, pH 3

Mobile Phase B:

90/10 MeCN/H₂O with 10 mM NH₄COOH and 0.125% HCOOH, pH 3

Flow Rate:

0.6 mL/min

Injection Volume:

5 μL (PLNO)

Sample Diluent:

75/25 MeCN/MeOH with 0.2% HCOOH

Column Temperature:

30 °C

Weak Needle Wash:

95/5 MeCN/H₂O

Instrument:

Waters ACQUITY UPLC with ACQUITY SQD

Ionization Mode:

ES+

Capillary:

3.0 KV

Cone:

30 V (6-Acetyl morphine and Morphine),

40 V (Morphine-3β-D-glucuronide)

Source Temperature:

120 °C

Desolvation Temperature:

350 °C

Desolvation Gas Flow (L/Hr):

800

SIR m/z:

329.5 (6-Acetyl morphine);

287.5 (Morphine)

463.6 (Morphine-3β-D-glucuronide)

Dwell Time:

0.1 s