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Desalting of Peptide Mixture Prior to ESI-MS Analysis

Desalting of Peptide Mixture Prior to ESI-MS Analysis

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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of peptide mixture.

Introduction

Experimental

EST-MS Conditions

Waters Micromass LCT System
Capillary voltage:	2.8 kV
Sample cone voltage:	35 V
Extraction cone voltage:	5 V
Gas flow:	520 and 310 L/h

Peptide mixture prepared by digestion of Cytochrome c by trypsin in 20 mM trisglycine buffer.

References

M. Gilar, A. Belenky, B. H. Wang, J. Chromatog. A, 921 (2001) 3–13.

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WA31764.66, June 2003