 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of sulfa drugs in serum.
The compounds used in this study are –
 
            
        | Column: | Symmetry C18, 3.9 x 150 mm, 5 μm | 
| Part number: | WAT046980 | 
| Mobile phase: | 1% glacial acetic acid and 4% methanol in water | 
| Flow rate: | 1 mL/min | 
| Injection volume: | 10 μL of reconstituted porcine serum extract | 
| Temperature: | 25 °C | 
| Detection: | UV @ 254 nm | 
 
            
         
            
         
            
        WA31764.142, June 2003