• Application Note

Naltrexone in Plasma - LC-MS

Naltrexone in Plasma - LC-MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates anaylsis of naltrexone in plasma by LC-MS.

Introduction

The compound analyzed in this study is naltrexone.

Experimental

HPLC Method

Column:

Symmetry C8, 2.1 x 30 mm, 5 μm

Part number:

WAT058977

Mobile phase:

10 mM NH4OH, pH 5/Acetonitrile 88:12

Flow rate:

200 μL/min

Injection volume:

50 μL plasma extract

MS:

Micromass Quattro II

Ion source temp.:

120 °C

CID:

1.8e-3 mBar, 20eV

Desolvation temp.:

400 °C

Nebulizer:

40 L/hr

Drying gas:

400 L/hr

Cone volt:

56V

Results and Discussion

CID mass spectra

Naltrexone m/z 342 > 324

Naloxone m/z 328 > 310

Coefficient of Determination: 0.996457

Calibration Curve: 0.0508208* x + 0.00306776

Response type: Internal Std (20 ng/mL Naloxone),

Area * ( IS Conc./IS Area)

Curve type: Linear, Origin: Exclude, Weighting: 1x, Axis trans: None

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WA31763.108, June 2003

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