• Application Note

Cocaine and its Metabolites in Serum

Cocaine and its Metabolites in Serum
  • Waters Corporation
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Plasma serum preparation in syringe

For forensic toxicology use only.

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of cocaine and its metabolites in serum using Symmetry C18 columns.

Introduction

Compounds analyzed in this application brief are:

1. Benzoylecgonine

2. Cocaine

3. Cocaethylene

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm (p/n: WAT046970)

Guard column:

Sentry guard column, 3.9 x 20 mm, 5 μm (p/n: WAT054250)

Mobile phase A:

20 mM potassium phosphate, pH 7

Mobile phase B:

Methanol

Flow rate:

1.0 mL/min

Injection volume:

1 mL of reconstituted porcine serum extract

Detection:

UV @ 235 nm

Gradient

Results and Discussion

Featured Products

WA31763.62, June 2003

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