• Application Note

Cytochrome C Tryptic Digest - pH 2.5, XTerra MS C18 

Cytochrome C Tryptic Digest - pH 2.5, XTerra MS C18 
  • Waters Corporation
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Cytochrome molecule structure

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of cytochrome C using XTerra MS C18 Columns.

Introduction

Cytochrome C has been analyzed in this study.

Experimental

HPLC Conditions

Column:

XTerra MS C18 4.6 x 50 mm, 3.5 μm (p/n: 186000432)

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM HCOOH, pH 2.5

Flow rate:

0.75 mL/min, 0.2 mL/min to MS

Injection volume:

50 μL (25 μg)

Temperature:

Ambient

Detection:

MS ESI+

Instrument:

Alliance 2695, Micromass ZQ

Gradient

MS Conditions

MS sytem:

Micromass ZQ

Ion source:

ESI+

Capillary:

3.5 kV

Cone:

30 V

Extractor:

3.0

Source temp.:

150 °C

Desolvation temp.:

400 °C

Cone gas flow:

60 L/hr

Desolvation gas flow:

550 L/hr

Results and Discussion

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WA20738.031, June 2002

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