UPLC-MS Analysis of Food Sugars/Saccharides in Beer Using ACQUITY UPLC BEH Amide Columns

Column:

ACQUITY UPLC BEH Amide 2.1 x 100 mm, 1.7 μm

Part Number:

186004801

Mobile Phase A:

80/20 MeCN/H₂O with 0.1% ammonium hydroxide [NH₄OH]

Mobile Phase B:

30/70 MeCN/H₂O with 0.1% ammonium hydroxide [NH₄OH]

Flow Rate:

0.13 mL/min

Gradient:

10 minute gradient, 75%-45% MeCN (w/0.1% NH₄OH) with 25 minute re-equilibration

Injection Volume:

2.0 μL (PLNO)

Sample Concentration:

Standards at 10 μg/mL, Beer at 50% dilution

Sample Diluent:

50/50 MeCN/H₂O

Column Temperature:

35 °C

Strong Needle Wash:

20/80 MeCN/H₂O (800 μL)

Weak Needle Wash:

75/2MeCN/H₂O 5 (500 μL)

Seal Wash:

50/50 MeCN/H₂O

Instrument:

Waters ACQUITY UPLC with SQ

Ionization Mode:

ES-

Capillary:

2.8 kV

Cone Voltage:

25 V (fructose, glucose, maltotriose);

40V (sucrose and maltose)

Source Temp:

120 °C

Desolvation Temp:

350 °C

Desolvation Gas Flow:

500 L/Hr

Cone:

50 L/Hr

SIR (m/z):

179.0 (fructose, glucose);

341.1 (sucrose, maltose);

503.2 (maltotriose)

Dwell Time:

0.04 s