• Application Note

Analysis of Nucleic Acid Bases Using Atlantis T3

Analysis of Nucleic Acid Bases Using Atlantis T3
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights analysis of nucleic acid bases

Introduction

Compounds analysed in this application brief :

  1. Cytosine
  2. 5-Fluorocytosine
  3. Uracil
  4. 5-Fluorouracil
  5. Guanine
  6. Thymine
  7. Adenine

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 150 mm, 5 ­m

Part Number:

186003747

Mobile Phase A:

H2O

Mobile Phase B:

ACN

Mobile Phase C:

100 mM CH3COONH4, pH 5.0

Flow Rate:

1.0 mL/min

Injection Volume:

10 μL

Column Temperature:

30˚C

Detection:

UV @ 254 nm

Instrument:

Waters Alliance 2695

Gradient

Time(min)

Profile

%A

%B

%C

0

90

0

10

10

84

6

10

11

90

0

10

15

90

0

10

Results and Discussion

Analysis of nucleic acid bases
1. Cytosine, 2. 5-Fluorocytosine, 3. Uracil, 4. 5-Fluorouracil, 5. Guanine, 6. Thymine, 7. Adenine.

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WA60208, January 2009

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