• Application Note

LC-MS Gradient Separation of 6-Acetylmorphine and Morphine on XBridge HILIC

LC-MS Gradient Separation of 6-Acetylmorphine and Morphine on XBridge HILIC
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the LC-MS gradient separation of 6-acetylmorphine and morphine on XBridge HILIC Columns.

Introduction

The compounds used in this study are:

  1. 6-Acetylmorphine
  2. Morphine

Experimental

LC Conditions

Column:

XBridge HILIC, 2.1 x 100 mm, 3.5 μm

Part number:

186004433

Mobile phase A:

10 mM NH4COOH with 0.125% formic acid in 50:50 ACN:H2O

Mobile phase B:

10 mM NH4COOH with 0.125% formic acid in 90:10 ACN:H2O

Flow rate:

0.6 mL/min

Sample concentration:

10 ng/mL each

Injection volume:

10.0 μL (PLNO, 20 μL loop)

Strong and weak needle wash:

95:5 ACN:H2O

Column temperature:

30 °C

Detection:

MS

Instrument:

Waters ACQUITY UPLC with TQD

Gradient:

Time(min)

Profile

%A

%B

0.00

0.1

99.9

1.05

0.1

99.9

4.35

99.9

0.1

4.50

0.1

99.9

6.00

0.1

99.9

MS Conditions

Ionization mode:

ES+

Capillary:

1.0 kV

Cone:

50 V

Source temperature:

120 °C

Desolvation temperature:

350 °C

Cone gas flow:

50 L/Hr

Desolvation gas flow:

800 L/Hr

Dwell time:

20 msec

ISD and ICD:

10 msec

MRM:

Morphine 286 > 200.9

6-Acetylmorphine 328 > 164.9

Results and Discussion

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