• Application Note

Isocratic Separation of Clonidine on Atlantis HILIC Silica

Isocratic Separation of Clonidine on Atlantis HILIC Silica
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the isocratic separation of clonidine on Atlantis HILIC Silica Columns.

Introduction

The compounds used in this study are:

  1. Clonidine

Experimental

LC Conditions

Column:

Atlantis HILIC Silica, 2.1 x 50 mm, 3 µm

Part Number:

186002011

Mobile Phase A:

200 mM NH4COOH, pH 3.0

Mobile Phase B:

ACN

Flow Rate:

0.3 mL/min

Isocratic Mobile Phase:

5% A; 95% B

Injection Volume:

5.0 µL

Sample Diluent:

75:25 ACN:MeOH

Sample Concentration:

10 ng/mL clonidine

Temperature:

Ambient

Instrument:

Waters Alliance HT System, 2795 Separations Module with Waters ZQ

MS Conditions

Ionization Mode:

ES+

Capillary:

2.0 kV

Cone:

40 V

Extractor:

3 V

RF Lens:

0.3 V

Source Temperature:

150 °C

Desolvation Temperature:

350 °C

Cone Gas Flow:

50 L/Hr

Desolvation Gas Flow:

700 L/Hr

SIR:

230.2 m/z

Results and Discussion

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