• Application Note

Analysis of Beta Blockers Using XBridge Shield RP18 

Analysis of Beta Blockers Using XBridge Shield RP18 
  • Waters Corporation
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Beta-blockers and statins

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of beta blockers using XBridge Shield RP18 Columns.

Introduction

Beta blockers are a class of drugs used for the management of cardiac arrhthmias, congestive heart failure, angina, and cardioprotection after mycardial infraction. They were once commonly prescribed for hypertension, before being replaced by more effective drugs.

Experimental

Test Conditions

Columns:

XBridge Shield RP18 4.6 X 100 mm, 3.5 μm p/n: 186003044

Mobile phase A:

H2O

Mobile phase B:

MeOH

Mobile phase C:

100 mM NH4HCO3, pH 9.0

Flow rate:

1.0 mL/min

Injection volume:

10 μL

Sample Concentration and Diluent:

10 μg/mL in H2O

Temp.:

30 °C

Sampling Rate:

5 points /second

Detection:

UV @ 280 nm

Time Constant:

1.0

Needle Wash:

5/95 MeOH/H2O

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time(min)

%A

%B

%C

0

85

5

10

3

55

35

10

10

55

35

10

11

85

5

10

15

85

5

10

System Suitability Parameters

Results and Discussion

Featured Products

WA60200, June 2007

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