• Application Note

Nalidixic Acid Antibiotics: SunFire Intelligent Speed Separation

Nalidixic Acid Antibiotics: SunFire Intelligent Speed Separation
  • Waters Corporation
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Pharmacist holding antibiotic capsule pack in pharmacy

Abstract

This application brief highlight the SunFire IS Column separation of antibiotics using MS detection.

Introduction

Compounds used in this study are shown below.

Experimental

Conditions

Column:

SunFire C18 2.1 x 20 mm IS, 3.5 μm (p/n: 186002531)

Mobile phase A:

Water

Mobile phase B:

MeOH

Mobile phase C:

1.0 % HCOOH in water

Flow rate:

0.4 mL/min

Injection volume:

2 μL

Sample concentration:

10 μg/mL in 50:50 Acetonitrile/ water

Temperature:

30 °C

Instrument:

Waters AutoPurification System

Gradient

ZQ Conditions

ES+

Capillary (kV):

3.5

Cone (V):

50

Extractor:

3

RF Lens:

0.1

Source temp. (oC):

150

Desolvation temp. (oC):

400

Cone Gas Flow (L/Hr):

50

Desolvation Gas Flow (L/Hr):

500

LM resolution:

15

HM Rrsolution:

15

Ion energy:

0.5

Multiplier (V):

650

Results and Discussion

Featured Products

WA41894, March 2005

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