• Application Note

Tetracyclines

Tetracyclines
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of tetracyclines using Symmetry C8 Columns.

Introduction

The compounds analyzed in this study are:

  1. Doxycycline 
  2. Minocycline 
  3. Tetracycline 
Compounds

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 μm

Guard column:

Symmetry Guard Column 3.9 x 20 mm, 5 μm

Part numbers:

Column - WAT046970, Guard - WAT054250

Mobile phase:

10 mM sodium phosphate, pH 6.25/methanol 45:55

Flow rate:

1.0 mL/min

Injection volume:

20 μL of 500 μg/mL sample

Detection:

UV @ 280 nm

USP Tailing Factors

  1. 1.05 
  2. 1.29 
  3. 1.14 

Results and Discussion

Results

Featured Products

WA31763.167, June 2003

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