• Application Note

Polar and Non-Polar Compounds Test Mix – 2.1 x 20 mm Intelligent Speed Separation

Polar and Non-Polar Compounds Test Mix – 2.1 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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Application Note

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of polar and non-polar compounds. 

Introduction

The compounds analyzed in this study are:

  1. 8-Bromoguanosine 
  2. Acetanilide 
  3. Triamcinolone 
  4. Hydrocortisone 
  5. 2-Amino-7-chloro-5-oxo-5H-[1]benzopyrano[2,3-b]pyridine-3-carbonitrile 
  6. 6α-Methyl-17α-hydroxyprogesterone 
  7. 3-Aminofluoranthene 
  8. 2-Bromofluorene 
  9. Perylene 
  10. Naphtho[2,3-a]pyrene 
Compounds

Experimental

Conditions

Columns:

Atlantis dC18, 2.1 x 20 mm IS, 3 µm, (P/N: 186002058)

Symmetry Shield RP18, 2.1 x 20 mm IS, 3.5 µm, (P/N: 186002068)

Symmetry C18, 2.1 x 20 mm IS, 3.5 µm, (P/N: 186002066)

XTerra MS C18, 2.1 x 20 mm IS, 3.5 µm, (P/N: 186001923)

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in Acetonitrile

Flow rate:

0.6 mL/min

Injection volume:

5 μL

Sample concentration:

20 μg/mL

Temperature:

30°C

Detection:

UV @ 254 nm

Instrument:

Alliance 2795 with 996 PDA

Gradient

Gradient

Results and Discussion

Results

Featured Products

WA31787.24, June 2003

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