• Application Note

Anilines, pH 10.0 – 4.6 x 20 mm Intelligent Speed Separation

Anilines, pH 10.0 – 4.6 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of anilines using XTerra Columns.  

Introduction

Experimental

Conditions

Column:

XTerra MS C18, 4.6 x 20 mm IS, 3.5 µm, (P/N: 186001891)

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

100 mM NH4HCO3, pH 10

Flow rate:

3.0 mL/min

Injection volume:

10 µL

Sample concentration:

20 µg/mL

Temperature:

Ambient

Detection:

UV @ 254 nm

Instrument:

Alliance 2795 with 996 PDA

Gradient

Time(min)

Profile

%A

%B

%C

0.0

90

0

10

4.0

60

30

10

Results and Discussion

The compounds analyzed in this study are:

  1. Caffeine
  2. Aniline
  3. N-Methylaniline
  4. 2-Ethylaniline
  5. 4-Nitroanisole
  6. N,N-Dimethylaniline 

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WA31787.10, June 2003

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