This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of hydrocortisone and related compound.
The compounds used in this study are –
Compounds |
USP Tailing Factors |
---|---|
Hydrocortisone |
1.1 |
Hydrocortisone-21-Acetate |
1.1 |
Column: |
Symmetry C18, 3.9 x 150 mm, 5 μm |
Part number: |
WAT046980 |
Mobile phase: |
Acetonitrile/water 35:65 |
Flow rate: |
1.0 mL/min |
Injection volume: |
10 μL of 10 μg/mL hydrocortisone and 30 μg/mL hydrocortisone-21-acetate |
Detection: |
UV @ 254 nm |
WA31763.89, June 2003