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Clemastine In Rat Plasma - Oasis On-Line 1 Column Approach

Clemastine In Rat Plasma - Oasis On-Line 1 Column Approach
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of clemastine In rat plasma using Oasis on-line 1 column approach method.

Introduction

Clemastine is studied in this application brief.

Experimental

Wash Conditions

LC1:

Alliance 2790 - 0.4 mL/min

LC2:

Waters 515–4.0 mL/min

Loading mobile phase:

100% water

Eluting mobile phase:

1 minute gradient 5% ACN to 95% ACN

Eluting mobile phase additive:

0.5% Formic acid

Extraction column temp.:

40 °C

Switching valve:

Rheodyne LabPro 10 ports, 2 position

MS:

Quattro Ultima Triple Quadrupole

Ion source:

ESI+

Source temp.:

150 °C

Gas cell:

1.5e-3 mbar

Desolvation gas:

600 L/hr

Cone voltage:

20 V

Collision energy:

20

Results and Discussion

Featured Products

WA31764.54, June 2003

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