This is an Application Brief and does not contain a detailed Experimental section.
This application brief details on the analysis of barbiturates using Symmetry Columns.
The compounds analyzed in this study are:
 
            
        | Column: | Symmetry C8, 3.9 x 150 mm, 5 µm | 
| Guard column: | Symmetry Guard Column 3.9 x 20 mm, 5 µm | 
| Part numbers: | Column - WAT046970, Guard - WAT054250 | 
| Mobile phase: | 100 mM Potassium phosphate, pH 6.9/acetonitrile/water 20:30:50 | 
| Flow rate: | 1.0 mL/min | 
| Injection volume: | 10 µL of 25µg/mL sample | 
| Detection: | UV @ 214 nm | 
 
            
        WA31763.33, June 2003