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Hydroxyisophthalic Acid

Hydroxyisophthalic Acid

  • Waters Corporation
Scientist analyzing pills in medical laboratory

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of hydroxyisophthalic acid using XTerra Columns. 

Introduction

The compound analyzed in this study is hydroxyisophthalic acid. 

Hydroxyisophthalic acid

Experimental

Conditions

Column:

XTerra RP18 4.6 x 150 mm, 5 µm

Part number:

186000492

Mobile phase:

pH 3.0: H2O/ACN/100 mM NH4COOH, pH 3.0 60:30:10

pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0 90:0:10

pH 10.0 H2O/ACN/100 mM NH4HCO3, pH 10.0 90:0:10

Flow rate:

1.0 mL/min

Injection volume:

5 µL of 250 µg/mL

Temperature:

30 °C

Detection:

UV @ 260 nm

Instrument:

Alliance 2695, 2996 PDA

Mobile Phase pH

USP Tailing

3.0

1.17

7.0

1.23

10.0

1.23

Results and Discussion

Results

WA20738.061, June 2002

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