This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of atenolol.
The compound analyzed in this study is atenolol.
Column: |
Xterra RP18 4.6 x 50 mm, 5 μm |
Part number: |
186000492 |
Mobile phase: |
pH 3.0: H2O/ACN/100 mM NH4COOH, pH 3.0 75:15:10 pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0 80:10:10 pH 10.0 H2O/ACN/100 mM NH4HCO3, pH 10.0 78:12:10 |
Flow rate: |
1.0 mL/min |
Injection volume: |
5 μL of 250 μg/mL |
Temperature: |
30 °C |
Detection: |
UV @ 270 nm |
Instrument: |
Alliance 2695, 2996 PDA |
Mobile Phase pH |
USP Tailing |
---|---|
3.0 |
1.21 |
7.0 |
1.38 |
10.0 |
1.06 |
WA20738.012, June 2002